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materials recombinant human cd5l protein  (R&D Systems)


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    R&D Systems materials recombinant human cd5l protein
    Figure 2. <t>CD5L</t> induced the expression of inflammatory factors in RA-FLS. The cells were stimulated with different concentrations of <t>recombinant</t> CD5L (0, 50, 100, 200, 500, 1000 ng/mL) for 24 h. (A) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. (C) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant after stimulating different time. (D) mRNA levels of IL-6, IL-8, and TNF-α after stimulating different time. The concentration of CD5L for stimulation was 500 ng/mL. Compared with the control group (0 ng/mL), ns, no statistical difference; *p < 0.05, **p < 0.01, ***p < 0.001. The measurements were repeated three times for each group of data.
    Materials Recombinant Human Cd5l Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials recombinant human cd5l protein/product/R&D Systems
    Average 93 stars, based on 5 article reviews
    materials recombinant human cd5l protein - by Bioz Stars, 2026-06
    93/100 stars

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    1) Product Images from "CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway."

    Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

    Journal: Autoimmunity

    doi: 10.1080/08916934.2023.2201412

    Figure 2. CD5L induced the expression of inflammatory factors in RA-FLS. The cells were stimulated with different concentrations of recombinant CD5L (0, 50, 100, 200, 500, 1000 ng/mL) for 24 h. (A) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. (C) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant after stimulating different time. (D) mRNA levels of IL-6, IL-8, and TNF-α after stimulating different time. The concentration of CD5L for stimulation was 500 ng/mL. Compared with the control group (0 ng/mL), ns, no statistical difference; *p < 0.05, **p < 0.01, ***p < 0.001. The measurements were repeated three times for each group of data.
    Figure Legend Snippet: Figure 2. CD5L induced the expression of inflammatory factors in RA-FLS. The cells were stimulated with different concentrations of recombinant CD5L (0, 50, 100, 200, 500, 1000 ng/mL) for 24 h. (A) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. (C) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant after stimulating different time. (D) mRNA levels of IL-6, IL-8, and TNF-α after stimulating different time. The concentration of CD5L for stimulation was 500 ng/mL. Compared with the control group (0 ng/mL), ns, no statistical difference; *p < 0.05, **p < 0.01, ***p < 0.001. The measurements were repeated three times for each group of data.

    Techniques Used: Expressing, Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Control

    Figure 3. CD5L activated ERK1/2 MAPK signaling pathway. Cells (5 × 106) were challenged with CD5L protein (500 ng/mL) for different times(0, 30, 60, 90 min). The protein was extracted and the expression levels of phosphorylation signal molecules (A) p-p38 MAPK, p-AKT, p-JAK, and p-I κB-α and (B) p-ERK were detected by WB.
    Figure Legend Snippet: Figure 3. CD5L activated ERK1/2 MAPK signaling pathway. Cells (5 × 106) were challenged with CD5L protein (500 ng/mL) for different times(0, 30, 60, 90 min). The protein was extracted and the expression levels of phosphorylation signal molecules (A) p-p38 MAPK, p-AKT, p-JAK, and p-I κB-α and (B) p-ERK were detected by WB.

    Techniques Used: Expressing, Phospho-proteomics

    Figure 4. Effect of inhibitor on CD5L-induced inflammatory-related factors in RA-FLS. The cells were pretreated with inhibitor U0126 for 1 h and cultured for 24 h with or without CD5L. The optimum concentration of U0126 deter mined by pre-experiment is 16 μM. (A) The expression levels of IL-6, IL-8, and TNF-α in the supernatant of cell culture were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. Results of three independent replicates were expressed as mean ± standard deviation. Figure 4. Continued.
    Figure Legend Snippet: Figure 4. Effect of inhibitor on CD5L-induced inflammatory-related factors in RA-FLS. The cells were pretreated with inhibitor U0126 for 1 h and cultured for 24 h with or without CD5L. The optimum concentration of U0126 deter mined by pre-experiment is 16 μM. (A) The expression levels of IL-6, IL-8, and TNF-α in the supernatant of cell culture were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. Results of three independent replicates were expressed as mean ± standard deviation. Figure 4. Continued.

    Techniques Used: Cell Culture, Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Figure 6. Effects of CD5L on apoptosis and proliferation of RA-FLS. Cells with inhibitor U0126 were pretreated for 1 h and cultured for 24 h with or without CD5L. (A) mRNA levels of BAX and BCL2 were detected by RT-PCR; (B) BAX and BCL-2 protein levels were detected by WB. (C) CCK-8 kit was used to detect the proliferation ability of RA-FLS. The blank wells contained only culture medium, and the control wells contained cells and culture medium. The exper imental results were expressed as mean ± standard deviation.
    Figure Legend Snippet: Figure 6. Effects of CD5L on apoptosis and proliferation of RA-FLS. Cells with inhibitor U0126 were pretreated for 1 h and cultured for 24 h with or without CD5L. (A) mRNA levels of BAX and BCL2 were detected by RT-PCR; (B) BAX and BCL-2 protein levels were detected by WB. (C) CCK-8 kit was used to detect the proliferation ability of RA-FLS. The blank wells contained only culture medium, and the control wells contained cells and culture medium. The exper imental results were expressed as mean ± standard deviation.

    Techniques Used: Cell Culture, Reverse Transcription Polymerase Chain Reaction, CCK-8 Assay, Control, Standard Deviation

    Figure 5. Effect of inhibitor on CD5L activation of ERK1/2 MAPK signaling pathway. Cells (5 × 106) were treated with ERK1/2 inhibitor U0126 for 1 h, and then added CD5L protein for 90 min. Protein was extracted, and the expression level of phosphorylated signal molecule p-ERK1/2 MAPK was detected by WB. GAPDH was used to correct the protein content of each sample.
    Figure Legend Snippet: Figure 5. Effect of inhibitor on CD5L activation of ERK1/2 MAPK signaling pathway. Cells (5 × 106) were treated with ERK1/2 inhibitor U0126 for 1 h, and then added CD5L protein for 90 min. Protein was extracted, and the expression level of phosphorylated signal molecule p-ERK1/2 MAPK was detected by WB. GAPDH was used to correct the protein content of each sample.

    Techniques Used: Activation Assay, Expressing



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    materials recombinant human cd5l protein  (R&D Systems)
    93
    R&D Systems materials recombinant human cd5l protein
    Figure 2. <t>CD5L</t> induced the expression of inflammatory factors in RA-FLS. The cells were stimulated with different concentrations of <t>recombinant</t> CD5L (0, 50, 100, 200, 500, 1000 ng/mL) for 24 h. (A) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. (C) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant after stimulating different time. (D) mRNA levels of IL-6, IL-8, and TNF-α after stimulating different time. The concentration of CD5L for stimulation was 500 ng/mL. Compared with the control group (0 ng/mL), ns, no statistical difference; *p < 0.05, **p < 0.01, ***p < 0.001. The measurements were repeated three times for each group of data.
    Materials Recombinant Human Cd5l Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials recombinant human cd5l protein/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    materials recombinant human cd5l protein - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

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    Figure 2. CD5L induced the expression of inflammatory factors in RA-FLS. The cells were stimulated with different concentrations of recombinant CD5L (0, 50, 100, 200, 500, 1000 ng/mL) for 24 h. (A) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. (C) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant after stimulating different time. (D) mRNA levels of IL-6, IL-8, and TNF-α after stimulating different time. The concentration of CD5L for stimulation was 500 ng/mL. Compared with the control group (0 ng/mL), ns, no statistical difference; *p < 0.05, **p < 0.01, ***p < 0.001. The measurements were repeated three times for each group of data.

    Journal: Autoimmunity

    Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

    doi: 10.1080/08916934.2023.2201412

    Figure Lengend Snippet: Figure 2. CD5L induced the expression of inflammatory factors in RA-FLS. The cells were stimulated with different concentrations of recombinant CD5L (0, 50, 100, 200, 500, 1000 ng/mL) for 24 h. (A) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. (C) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant after stimulating different time. (D) mRNA levels of IL-6, IL-8, and TNF-α after stimulating different time. The concentration of CD5L for stimulation was 500 ng/mL. Compared with the control group (0 ng/mL), ns, no statistical difference; *p < 0.05, **p < 0.01, ***p < 0.001. The measurements were repeated three times for each group of data.

    Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

    Techniques: Expressing, Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Control

    Figure 3. CD5L activated ERK1/2 MAPK signaling pathway. Cells (5 × 106) were challenged with CD5L protein (500 ng/mL) for different times(0, 30, 60, 90 min). The protein was extracted and the expression levels of phosphorylation signal molecules (A) p-p38 MAPK, p-AKT, p-JAK, and p-I κB-α and (B) p-ERK were detected by WB.

    Journal: Autoimmunity

    Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

    doi: 10.1080/08916934.2023.2201412

    Figure Lengend Snippet: Figure 3. CD5L activated ERK1/2 MAPK signaling pathway. Cells (5 × 106) were challenged with CD5L protein (500 ng/mL) for different times(0, 30, 60, 90 min). The protein was extracted and the expression levels of phosphorylation signal molecules (A) p-p38 MAPK, p-AKT, p-JAK, and p-I κB-α and (B) p-ERK were detected by WB.

    Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

    Techniques: Expressing, Phospho-proteomics

    Figure 4. Effect of inhibitor on CD5L-induced inflammatory-related factors in RA-FLS. The cells were pretreated with inhibitor U0126 for 1 h and cultured for 24 h with or without CD5L. The optimum concentration of U0126 deter mined by pre-experiment is 16 μM. (A) The expression levels of IL-6, IL-8, and TNF-α in the supernatant of cell culture were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. Results of three independent replicates were expressed as mean ± standard deviation. Figure 4. Continued.

    Journal: Autoimmunity

    Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

    doi: 10.1080/08916934.2023.2201412

    Figure Lengend Snippet: Figure 4. Effect of inhibitor on CD5L-induced inflammatory-related factors in RA-FLS. The cells were pretreated with inhibitor U0126 for 1 h and cultured for 24 h with or without CD5L. The optimum concentration of U0126 deter mined by pre-experiment is 16 μM. (A) The expression levels of IL-6, IL-8, and TNF-α in the supernatant of cell culture were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. Results of three independent replicates were expressed as mean ± standard deviation. Figure 4. Continued.

    Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

    Techniques: Cell Culture, Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Figure 6. Effects of CD5L on apoptosis and proliferation of RA-FLS. Cells with inhibitor U0126 were pretreated for 1 h and cultured for 24 h with or without CD5L. (A) mRNA levels of BAX and BCL2 were detected by RT-PCR; (B) BAX and BCL-2 protein levels were detected by WB. (C) CCK-8 kit was used to detect the proliferation ability of RA-FLS. The blank wells contained only culture medium, and the control wells contained cells and culture medium. The exper imental results were expressed as mean ± standard deviation.

    Journal: Autoimmunity

    Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

    doi: 10.1080/08916934.2023.2201412

    Figure Lengend Snippet: Figure 6. Effects of CD5L on apoptosis and proliferation of RA-FLS. Cells with inhibitor U0126 were pretreated for 1 h and cultured for 24 h with or without CD5L. (A) mRNA levels of BAX and BCL2 were detected by RT-PCR; (B) BAX and BCL-2 protein levels were detected by WB. (C) CCK-8 kit was used to detect the proliferation ability of RA-FLS. The blank wells contained only culture medium, and the control wells contained cells and culture medium. The exper imental results were expressed as mean ± standard deviation.

    Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

    Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, CCK-8 Assay, Control, Standard Deviation

    Figure 5. Effect of inhibitor on CD5L activation of ERK1/2 MAPK signaling pathway. Cells (5 × 106) were treated with ERK1/2 inhibitor U0126 for 1 h, and then added CD5L protein for 90 min. Protein was extracted, and the expression level of phosphorylated signal molecule p-ERK1/2 MAPK was detected by WB. GAPDH was used to correct the protein content of each sample.

    Journal: Autoimmunity

    Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

    doi: 10.1080/08916934.2023.2201412

    Figure Lengend Snippet: Figure 5. Effect of inhibitor on CD5L activation of ERK1/2 MAPK signaling pathway. Cells (5 × 106) were treated with ERK1/2 inhibitor U0126 for 1 h, and then added CD5L protein for 90 min. Protein was extracted, and the expression level of phosphorylated signal molecule p-ERK1/2 MAPK was detected by WB. GAPDH was used to correct the protein content of each sample.

    Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

    Techniques: Activation Assay, Expressing